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- Title
Two different proteases produced by a deep-sea psychrotrophic bacterial strain, Pseudoaltermonas sp. SM9913.
- Authors
Chen, Xiu-Lan; Zhang, Yu-Zhong; Gao, Pei-Ji; Luan, Xi-Wu
- Abstract
A psychrotrophic bacterial strain, Pseudoaltermonas sp. SM9913, was isolated from deep-sea sediment collected at 1,855 m depth. Two proteases produced by Pseudoaltermonas sp. SM9913 were purified, MPC-01 and MCP-02. MCP-01 is a serine protease with a molecular weight of 60.7 kDa. It is cold-adapted with an optimum temperature of 30–35°C. Its Km and Ea for the hydrolysis of casein were 0.18% and 39.1 kJ mol-1, respectively. It had low thermostability, and its activity was reduced by 73% after incubation at 40°C for 10 min. MCP-02 is a mesophilic metalloprotease with a molecular weight of 36 kDa. Its optimum temperature for the hydrolysis of casein was 50–55°C. The Km and Ea of MCP-02 for the hydrolysis of casein were 0.36% and 59.3 kJ mol-1, respectively. MCP-02 had high thermostability, and its activity was reduced by only 30.5% after incubation at 60°C for 10 min. At low temperatures, Pseudoaltermonas sp. SM9913 mainly produced the psychrophilic protease MCP-01.
- Subjects
PROTEOLYTIC enzymes; HYDROLYSIS; MILK proteins; CASEINS; CRYOSCOPY; MOLECULAR weights; AMINO acids
- Publication
Marine Biology, 2003, Vol 143, Issue 5, p989
- ISSN
0025-3162
- Publication type
Article
- DOI
10.1007/s00227-003-1128-2