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- Title
Analysis of benzalkonium chloride and benzethonium chloride in cosmetics by HPLC.
- Authors
Qilin Wang; Xiaoping Han; Zhenbing Li; Qikui Tang
- Abstract
A method for simultaneous determination of benzalkonium chloride and benzethonium chloride in cosmetics was established by high performance liquid chromatography. Different types of chromatographic columns and mobile phase conditions were screened. Finally, C8 column was used to separate benzethonium chloride and three kinds of homologues of dimethyl benzyl ammonium chlorides calculated with benzalkonium chloride. Methanol : 0.1 mol/L ammonium acetate buffer solution( pH=5) was used as the mobile phase for isocratic elution. The results were simultaneously determined by DAD at the wavelength of 260 nm. Compared with the methods recommended in Technical Specification for Safety of Cosmetics( 2015 edition), the detection method of benzalkonium chloride and the detection method of lauralkonium chloride, benzethonium chloride and cetalkonium chloride were optimized and combined. A C8 column with a wide range of use was selected to achieve the simultaneous separation of the four components. Benzalkonium chloride and benzethonium chloride have good linear relationship in a certain mass concentration range, and the correlation coefficients are greater than 0.999 9. The recoveries were 90%-110% with RSD of 0.3%-4.4% (n=9) at three mass concentration gradients of low, medium and high for four different types of matrixes. This method has the advantages of simple operation, short analysis time, good sensitivity and reproducibility, and can be used for the simultaneous determination of benzalkonium chloride and benzethonium chloride in cosmetics.
- Subjects
BENZALKONIUM chloride; HIGH performance liquid chromatography; AMMONIUM chloride; BENZYL chloride; CHLORIDES; COSMETICS
- Publication
China Surfactant Detergent & Cosmetics (1001-1803), 2022, Vol 52, Issue 9, p1023
- ISSN
1001-1803
- Publication type
Article
- DOI
10.3969/j.issn.1001-1803.2022.09.016