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- Title
不同毛色小鼠背部皮肤中甘油二酯激酶ζ 和蛋白激酶Cβ Ⅱ的表达和定位.
- Authors
李 静; 谢建山; 崔慧林; 曹锡梅; 杨艳萍; 李海荣
- Abstract
BACKGROUND: In the skin and hair pigmentation mechanism, diacylglycerol kinase ζ (DGKζ) has not been reported to participate in pigmentation. OBJECTIVE: To investigate the expression and localization of DGKζ and protein kinase C βII (PKCβII) in the mouse back skin with different coat colors and their correlation with the formation of coat color. METHODS: The back skin of 2-month-old mice with different coat colors (white, gray, and black, 6 mice for each color) was taken to detect DGKζ and PKCβII mRNAs by real-time PCR, DGKζ, PKCβII and p-PKCβII proteins by western blot, and DGKζ and p-PKCβII expression by immunohistochemistry. The study protocol was approved by the Animal Ethics Committee of the Animal Experimental Center of Shanxi Medical University. RESULTS AND CONCLUSION: DGKζ and PKCβII mRNAs were both expressed in the back skin of mice with three kinds of coat colors, with its relative expression amount being: gray group > white group; black group > white group (P < 0.05). Both DGKζ and PKCβII proteins were expressed in the back skin of mice with different hair coat colors. The ratio of p-PKCβII to PKCβII showed that the change of PKCβII activity had the same tendency with DGKζ expression: gray group > white group; black group > white group (P < 0.05). Immunohistochemistry staining showed that DGKζ and p-PKCβII were positively expressed in the outer root sheath of hair follicle, hair matrix cells and melanocytes in the hair matrix in the back skin of gray and black mice, while were negative in the hair follicles of white mice. According to the expression and localization of DGKζ, PKCβII in white, gray and black mouse back skin, we can speculate that DGKζ and PKCβII may participate in the formation of the coat color.
- Subjects
PROTEIN kinase C; ANIMAL coloration; HAIR cells; WESTERN immunoblotting; ACADEMIC medical centers; HUMAN skin color; HAIR follicles
- Publication
Chinese Journal of Tissue Engineering Research / Zhongguo Zuzhi Gongcheng Yanjiu, 2021, Vol 25, Issue 8, p1196
- ISSN
2095-4344
- Publication type
Article
- DOI
10.3969/j.issn.2095-4344.3038