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- Title
肝血窦内皮细胞介导的他莫昔芬对非酒精性脂肪性肝炎治疗作用 的转录组学分析.
- Authors
房志强; 阮柏; 刘晶晶; 许皓; 王琳
- Abstract
Objective: To determine the effect of tamoxifen on the metabolism, inflammation and fibrosis-related gene expression of LSECs in a diet-induced NASH mouse model. Methods: 8-week-old male C57BL/6 mice were purchased from Charles River. Then these mice were fed MCD diets for 6 weeks and administrated tamoxifen intraperitoneally (100 mg/kg/day) for five consecutive days. The next day, these mice were anesthetized and LSECs were isolated and suspended by 1 ml TRIzol Reagent and placed at-80℃ refrigerator. The samples were then sent to Genedenovo Biotechnology for RNA sequencing and subsequent bioinformatic analysis were performed on Omicsmart platform. We also reanalyzed others’ sequencing data to support our evidence using Omicshare tools. Results: Through analyzing our own RNA-seq data and reanalyzing Xiong X’s RAN-seq data, combined with liver ECs-specific metabolic gene sets derived from Joanna Kalucka et al, we found that compared to normal LSECs, inflammation and fibrosis-related gene expression was upregulated notably. These effects were abolished by tamoxifen administration. Meanwhile, GSEA analysis suggested that tamoxifen downregulated the expression of inflammation and fibrosis-related pathway. Conclusions: Tamoxifen reversed the alterations of metabolic genes of LSECs and up-regulated genes related to inflammation and fibrosis in mice with nonalcoholic steatohepatitis.
- Subjects
NON-alcoholic fatty liver disease; RNA sequencing; GENE expression; LABORATORY mice; TAMOXIFEN
- Publication
Progress in Modern Biomedicine, 2022, Vol 22, Issue 9, p1609
- ISSN
1673-6273
- Publication type
Article
- DOI
10.13241/j.cnki.pmb.2022.09.002