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- Title
褪黑素通过改善神经元树突棘可塑性在瑞芬太尼诱发大鼠痛觉过敏机制中的作用.
- Authors
吕晓敏; 陈红; 薛朝霞; 王丽斯; 刘奇
- Abstract
Objective: To explore the role of melatonin in the mechanism of remifentanil-induced hyperalgesia in rats by improving the plasticity of neuronal dendritic spines. Methods: Purchase 30 SD rats (175~200 g) aged 8-10 weeks. According to the purpose of the study, all rats were randomly divided into three groups: control group (rats undergoing incision surgery on the soles of rats, treated with saline, n=10), model group (rats undergoing incisions on the soles of rats, sutured intravenously remifentanil, n=10), treatment group (melatonin treatment on the basis of the model group, n=10). Shrinkage feet reflection of rats in different groups at 2 h, 2 d, 3 d and 7 d after treatment was detected by the paw withdrawal threshold and the paw withdrawal latency time. The mRNA expression of IL-1, TNF-α and IL-1β in rats was analyzed by RT-qPCR. The protein expression of AMPA and Kalirin-7 in rats was detected by Western blot. The heat sensitivity of rats was evaluated by a hot-plate biological instrument. The density and length of the spine of rats in different groups were compared by observing tissue sections. The electrophysiology of a single neuron is analyzed by the camera monitor of a low-sensitivity charge-coupled device. Results: In the 2 h, 2 d, 3 d and 7 d tests, comparing with the control group, the paw withdrawal threshold was lower (P<0.05), the paw withdrawal latency time was shorter (P<0.05), the mRNA expression of IL-1, TNF-α, IL-1β and the protein expression of AMPA and Kalin-7 was higher (P<0.05) in the model group. And the spine density and length and the current amplitude was higher (P<0.05), the current interval was lower (P<0.05) in the model group. Comparing with the model group, the paw withdrawal threshold was higher(P<0.05), the paw withdrawal latency time was longer(P<0.05), the mRNA expression of IL-1, TNF-α, IL-1βand the protein expression of AMPA and Kalin-7 was lower (P<0.05) in the treatment group. And the spine density and length and the current amplitude was lower (P<0.05), the current interval was increased (P<0.05) in the model group. Conclusion: Melatonin improves the plasticity of neuronal dendritic spines by inhibiting the protein expression of AMPA and Kalirin-7, thereby reducing remifentanil- induced hyperalgesia in rats.
- Subjects
RATS; DENDRITIC spines; INTERLEUKIN-1; SURGICAL site; PROTEIN expression; WESTERN immunoblotting
- Publication
Progress in Modern Biomedicine, 2021, Vol 21, Issue 9, p1633
- ISSN
1673-6273
- Publication type
Article
- DOI
10.13241/j.cnki.pmb.2021.09.007