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- Title
桂皮醛联合万古霉素对耐甲氧西林金黄色葡萄球菌生物膜的抑制作用.
- Authors
张雅娟; 潘红; 杜佳慧; 吴洁; 邱秀芹; 刘松柏; 贾蓓蓓
- Abstract
Objective To investigate the effect of cinnamaldehyde and vancomycin on Biofilm inhibition of methicillin-resistant staphylococcus aureus(MRSA). Methods Modified plate culture method was used to establish in vitro biofilm model for MRSA standard strain ATCC25923 and other 5 strains from patient samples,and crystal violet semi-quantitative method was applied to detect the biofilm formation. The minimum inhibitory concentration (MIC) of ATCCA strain and typical MRSA strain was determined using triphenyl tetrazolium chloride (TTC) method. Micrdilution checkerboard techniques was adopted to determine the MIC of drugs combination. Results The biofilmwill be maturated in three days;Strain 16187 showed strong capacity of biomilm formation;MICs of standard strain for cinnamaldehyde and vancomycin were 256 µg/mL and 1 µg/mL;MICs of strain 16187 for cinnamaldehyde and vancomycin were 128 µg/mL and 0.5 µg/mL;MIC of standard strain for combination of cinnamaldehyde and vancomycin were 16 µg/mL and 0.006 µg/mL,and 16 µg/mL and 0.03 µg/mL for stain 16187. Comparing MRSA16187 in treated with medicatoin conbired with cinnamaldehyde and vancomycin and single medication the difference of sMIC50 was significant (p<0.05 andp<0.05),and there was significant difference when we compared sMIC90 (p<0.05 andp<0.05);Comparing ATCC25923 treated with medication combined with cinnamaldehyde and vancomycin the difference was of sMIC50 significant (p<0.05 andp<0.05), and sMIC90 showed the significant difference (p<0.05 andp<0.01) .Conclusion Cinnamaldehyde synergistically enhances the capacity of vancomycin on biofilminhibitory on MRSA.
- Publication
Journal of Kunming Medical University / Kunming Yike Daxue Xuebao, 2018, Vol 39, Issue 4, p52
- ISSN
2095-610X
- Publication type
Article