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- Title
PPAR-γ agonist ameliorates liver pathology accompanied by increasing regulatory B and T cells in high-fat-diet mice.
- Authors
Xu, Zhipeng; Wang, Gang; Zhu, Yuxiao; Liu, Ran; Song, Jingwei; Ni, Yangyue; Sun, Hongzhi; Yang, Bingya; Hou, Min; Chen, Lin; Ji, Minjun; Fu, Zan
- Abstract
<bold>Objective: </bold>Peroxisome proliferator-activated receptor (PPAR)-γ plays critical roles in human metabolic disorders. However, the mechanism remains incompletely understood. Regulatory cells contribute to these metabolic improvements; therefore, whether PPAR-γ agonist regulates regulatory cells was investigated.<bold>Methods: </bold>C57BL/6J mice received a normal or high-fat diet (HFD) with or without pioglitazone treatment. Mice were sacrificed for detecting the metabolic parameters. Lymphocytes from spleen and visceral adipose tissue (VAT) were collected and analyzed for ST2+ Tregs and Bregs by flow cytometry. IL-10 in the liver or VAT was detected by immunofluorescence and ELISA. Correlation analysis between IL-10 and liver weight or serum total cholesterol was made by Pearson correlation analysis.<bold>Results: </bold>Pioglitazone increased VAT weight but reduced serum total cholesterol, hepatic steatosis, and cholesterol crystallization formation. Pioglitazone treatment enhanced ST2+ Tregs and Bregs in the VAT and spleen of HFD-fed mice (all P < 0.05). Pioglitazone treatment increased IL-10 in the livers or VAT of HFD-fed mice (all P < 0.05). The expression of IL-10 in the liver was significantly negatively correlated with liver weight or serum total cholesterol in pioglitazone-treated HFD-fed mice (r2 = 0.74, P < 0.05; r2 = 0.58, P < 0.05).<bold>Conclusions: </bold>PPAR-γ signaling plays a critical role in the regulation of metabolic disorders through promoting regulatory cell response.
- Subjects
ENZYME-linked immunosorbent assay; PREVENTIVE medicine; T cell differentiation; FATTY liver; OBESITY treatment; DIAGNOSIS; DISEASE risk factors; PROTEIN metabolism; ADIPOSE tissues; ANIMAL experimentation; B cells; CHOLESTEROL; DIET; INTERLEUKINS; LIVER; MICE; PROTEINS; T cells; PHARMACODYNAMICS; THIAZOLIDINEDIONES; THERAPEUTICS
- Publication
Obesity (19307381), 2017, Vol 25, Issue 3, p581
- ISSN
1930-7381
- Publication type
journal article
- DOI
10.1002/oby.21769