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- Title
Screening and Identification of Saline-alkali Tolerant and Tobacco Black Shank Resistant Trichoderma.
- Authors
CUI Xi-ling; LI Shi-gui; YANG Jia; FAN Xiang-chen; GU Jin-gang
- Abstract
In this study, 20 saline-alkali tolerant Trichoderma strains were isolated and screened from 48 saline-alkali soil samples collected in Tianjin, Hebei Province by dilution plate coating method. When they were cultured on PDA medium at 8% NaCl concentration and pH 8.0 for 7 d, colony diameter went up to 1 . 0 - 5 . 9 cm. Phytophthora nicotianae ACCC38065 was used as pathogen to evaluate the disease-resistant effect of Trichoderma selected. Six strains were selected, which have better antagonistic effect on Phytophthora nicotianae ACCC38065. Of which, strain 28TJ19 has the best antagonism effect. Tablet confrontation and inhibition rate were 52.8% and 71.6%, respectively, followed by strain 28HB14 (41.7%, 76.2%), 29HB16(41.7%, 67.3%). These six strains were classified and identified by morphological observation, ITS sequence analysis and TEF sequence analysis. Four of them were identified as Trichoderma harzianum. The other two strains were Trichoderma longibrachiatum. The results of greenhouse test indicated that all these 6 strains had bio-control effect of different degrees on tobacco blank shank. Among them the effect of strain 29TJ04 and 29HB16 were the best, which could reach 100%.
- Subjects
TRICHODERMA; PHYTOPHTHORA nicotianae; TOBACCO diseases &; pests; PHYSIOLOGICAL control systems; PHYTOPATHOGENIC microorganisms
- Publication
Journal of Agricultural Science & Technology (1008-0864), 2014, Vol 16, Issue 3, p81
- ISSN
1008-0864
- Publication type
Article
- DOI
10.13304/j.nykjdb.2013.450