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- Title
Burkholderia contaminans Biofilm Regulating Operon and Its Distribution in Bacterial Genomes.
- Authors
Voronina, Olga L.; Kunda, Marina S.; Ryzhova, Natalia N.; Aksenova, Ekaterina I.; Semenov, Andrey N.; Romanova, Yulia M.; Gintsburg, Alexandr L.
- Abstract
Biofilm formation by Burkholderia spp. is a principal cause of lung chronic infections in cystic fibrosis patients. A “lacking biofilm production” (LBP) strain B. contaminans GIMC4587:Bct370-19 has been obtained by insertion modification of clinical strain with plasposon mutagenesis. It has an interrupted transcriptional response regulator (RR) gene. The focus of our investigation was a two-component signal transduction system determination, including this RR. B. contaminans clinical and LBP strains were analyzed by whole genome sequencing and bioinformatics resources. A four-component operon (BiofilmReg) has a key role in biofilm formation. The relative location (i.e., by being separated by another gene) of RR and histidine kinase genes is unique in BiofilmReg. Orthologs were found in other members of the Burkholderiales order. Phylogenetic analysis of strains containing BiofilmReg operons demonstrated evidence for earlier inheritance of a three-component operon. During further evolution one lineage acquired a fourth gene, whereas others lost the third component of the operon. Mutations in sensor domains have created biodiversity which is advantageous for adaptation to various ecological niches. Different species Burkholderia and Achromobacter strains all demonstrated similar BiofilmReg operon structure. Therefore, there may be an opportunity to develop a common drug which is effective for treating all these causative agents.
- Subjects
DNA analysis; BIOFILMS; CELLULAR signal transduction; CYSTIC fibrosis; BIOLOGICAL evolution; GENETICS; GENETIC techniques; GENOMES; LUNGS; GENETIC mutation; RESEARCH funding; BIOINFORMATICS; MAXIMUM likelihood statistics; BURKHOLDERIA; BURKHOLDERIA infections; DATA analysis software; SEQUENCE analysis; DISEASE complications
- Publication
BioMed Research International, 2016, Vol 2016, p1
- ISSN
2314-6133
- Publication type
Article
- DOI
10.1155/2016/6560534