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- Title
Two-dimensional protein profiles of palisade parenchyma and of spongy parenchyma of Vicia faba leaf.
- Authors
Liming Shen; Outlaw Jr., William H.
- Abstract
Specific probes such as targeted-enzyme assays have failed to reveal qualitative differences between the metabolism of spongy parenchyma and that of palisade parenchyma cells. To determine whether the results from these definite assays support a general conclusion concerning the overall biochemical similarity of these cells, two-dimensional protein profiles have been constructed for these two types of cells. Protein extracts were prepared from freeze-dried homogeneous tissue samples or from isolated protoplasts of Vicia faba L. cv. Longpod leaves. Proteins separated by micro two-dimensional gel electrophoresis were stained with silver. The relative apparent abundance (based on visual judgment of the area and intensity of staining) was estimated for each spot. Pairwise comparison of nominally 125 stained proteins of each of approximately 75 gel pairs did not reveal a consistent qualitative difference between profiles. Recognizing that polypeptides escaped detection in our investigation through loss, failure to stain sufficiently, or incomplete resolution, we qualify our conclusion that spongy parenchyma and palisade are similar in their overall protein complement.
- Subjects
PLANT parenchyma; ELECTROPHORESIS; FAVA bean; HISTOCHEMISTRY; PLANT enzymes; BOTANY
- Publication
Physiologia Plantarum, 1989, Vol 77, Issue 4, p599
- ISSN
0031-9317
- Publication type
Article
- DOI
10.1111/j.1399-3054.1989.tb05397.x