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- Title
MiR-429-5p attenuates the migration and invasion of malignant melanoma by targeting LIMK1.
- Authors
H. SHENG; Y.-H. GUO; D.-S. CAO; X.-J. LI; Y. ZHAO; H. DING; Y.-L. ZHANG; Z.-H. CHEN
- Abstract
OBJECTIVE: To investigate the potential effects of microRNA-429-5p (miR-429-5p) on the development of malignant melanoma (MM) and the relevant mechanism. PATIENTS AND METHODS: Quantitative Real Time-Polymerase Chain Reaction (qRT-PCR) was used to detect the differential expression of miR-429-5p in MM tissues. The relationship between miR-429-5p expression and clinical pathological data of MM patients was analyzed. LIM kinase 1 (LIMK1) was verified as a downstream target of miR-429-5p by online prediction software, and the interaction between LIMK1 and miR-429-5p was verified by Dual-Luciferase reporter assay. RESULTS: Compared with normal skin tissues, miR-429-5p was downregulated in MM tissues. MiR-429-5p expression was correlated with tumor size and stage of MM. Upregulation of miR-429-5p significantly inhibited protein expression of LIMK1 and reduced migration and invasion ability of MM cells. LIMK1 was involved in MM progression regulated by miR-429-5p. CONCLUSIONS: MiR-429-5p attenuates migration and invasion in MM by targeting LIMK1. Hence, miR-429-5p/LIMK1 axis might be a potential therapeutic target for the treatment of MM.
- Subjects
MELANOMA; MILITARY invasion; TUMOR classification; PROTEIN expression; FORECASTING; QUANTITATIVE research
- Publication
European Review for Medical & Pharmacological Sciences, 2020, Vol 24, Issue 5, p2625
- ISSN
1128-3602
- Publication type
Article