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- Title
Long form collapsin response mediator protein-1 promotes the migration and invasion of osteosarcoma cells.
- Authors
HUIGE HOU; LIN CHEN; ZHENGANG ZHA; SHAOHUI CAI; MINGHUI TAN; GUOQING GUO; NING LIU; GUORONG SHE; SONGWEI XUN
- Abstract
It has been reported that long form collapsin response mediator protein-1 (LCRMP-1) promotes the metastasis of non-small cell lung cancer. Osteosarcoma (OS) is a human cancer with a high potential for metastasis. The present study aimed to investigate the role of LCRMP-1 in OS metastasis. The expression of LCRMP-1 in OS specimens and cell lines was evaluated using reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and western blot analysis. Furthermore, the migration and invasion of OS cells with LCRMP-1-knockdown was investigated to examine the role of LCRMP-1 in OS metastasis. In addition, the expression of N-cadherin and matrix metalloproteinases (MMPs), which are involved in cell migration, was evaluated using RT-qPCR. Increased expression of LCRMP-1 was observed in the OS tissues and cell lines, accompanied by the enhanced migration and invasion of the OS cells. LCRMP-1-knockdown resulted in a significant decrease in the expression of N-cadherin and MMPs, as well as inhibition of the migration and invasion of the OS cells. Overexpression of LCRMP-1 promoted OS metastasis. Therefore, LCRMP-1 may be a promising target for the effective treatment of OS.
- Subjects
COLLAPSINS; LUNG cancer &; genetics; OSTEOSARCOMA; LUNG cancer risk factors; POLYMERASE chain reaction; METALLOPROTEINASES; DIAGNOSIS
- Publication
Oncology Letters, 2016, Vol 12, Issue 1, p23
- ISSN
1792-1074
- Publication type
Article
- DOI
10.3892/ol.2016.4562