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- Title
Rapid changes in cell wall pectic polysaccharides are closely associated with early stages of aerenchyma formation, a spatially localized form of programmed cell death in roots of maize (Zea mays L.) promoted by ethylene.
- Authors
Gunawardena, A. H. L. A. N.; Pearce, D. M. E.; Jackson, M. B.; Hawes, C. R.; Evans, D. E.
- Abstract
Abstract Aerenchyma formation in roots of maize (Zea mays L.) involves programmed death of cortical cells that is promoted by exogenous ethylene (1 µL L-1) or by endogenous ethylene produced in response to external oxygen shortage (3%, v/v). In this study, evidence that degeneration of the cell wall accompanies apoptotic-like changes previously observed in the cytoplasm and nucleus (Gunawardena et al. Planta 212, 205–214, 2001), has been sought by examining de-esterified pectins (revealed by monoclonal antibody JIM 5), and esterified pectins (revealed by monoclonal antibody JIM 7). In controls, de-esterified wall pectins were found at the vertices of triangular junctions between cortical cells (untreated roots). Esterified pectins in control roots were present in the three walls bounding triangular cell-to-cell junctions. After treatment with 3% oxygen or 1 µL L-1 ethylene, this pattern was lost but walls surrounding aerenchyma gas spaces became strongly stained. The results showed that cell wall changes commenced within 0·5 d and evidently were initiated by ethylene in parallel with cytoplasmic and nucleoplasmic events associated with classic intracellular processes of programmed cell death.
- Subjects
CORN root physiology; PLANT cell walls; PECTINS; APOPTOSIS; EFFECT of ethylene on plants
- Publication
Plant, Cell & Environment, 2001, Vol 24, Issue 12, p1369
- ISSN
0140-7791
- Publication type
Article
- DOI
10.1046/j.1365-3040.2001.00774.x