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- Title
Identification of the Gram Positive Bacterial Sepsis Agent with Rapid Genotype Test.
- Authors
Erdogan, Ziya; Ozturk, Cihadiye Elif; Cangur, Sengul; Caliskan, Emel; Oksuz, Sukru; Karamurat, Zeynep Dilara
- Abstract
Objective: An irreversible process begins when a systemic infection causes sepsis. Therefore, rapid identification of the agent bacteria in sepsis and its antibiotic resistance is crucially important. In this study, it was aimed to investigate the efficiency of rapid genotype test in detecting sepsis agent Gram positive bacteria and important antibiotic resistance. Methods: 2132 blood culture samples sent to the laboratory were examined with an automatic blood culture system (BACTEC, BD, USA) between 2018-2019. Blood culture bottles sent to the laboratory were Growing bacteria was identificated by VİTEK (bioMérieux, France) automated bacteria identification / antibiotic susceptibility system. In addition, bacterial species and mecA, vanA, vanB, vanC1, vanC2 / C3 genes in blood cultures with Gram positive bacterial growth were also determined by the "Genotype® BC Gram-positive (Hain Lifesience, Germany)" test. Results: 72 patients with gram-positive bacteria growth in two or more blood culture bottles were included in the study. In 44 of the samples (61%) the same bacterial species were detected with conventional method (bacteria culture) and BC Gram positive test. In 28 of the samples (39%) differences were detected between results of methods regarding bacterial species name or vancomycin/methicillin resistance rate. Although single agent was isolated with culture method in all of the samples, multiple agents were detected in eight samples with rapid genotype test. Also, it was found that in mecA positive samples, ciprofloxacin resistance was higher than mecA negative ones. Conclusions: In the study, it was observed that BC Gram positive test could correctly identify sepsis agent bacteria and their resistance genes within 4-5 hours.
- Subjects
TURKEY; BLOOD; CLINICAL pathology; CELL culture; CIPROFLOXACIN; VANCOMYCIN; SEPSIS; GRAM-positive bacterial infections; GENOTYPES; GENES; DESCRIPTIVE statistics; DRUG resistance in microorganisms; METHICILLIN; GRAM-positive bacteria
- Publication
Konuralp Medical Journal / Konuralp Tip Dergisi, 2021, Vol 13, Issue 3, p511
- ISSN
1309-3878
- Publication type
Article
- DOI
10.18521/ktd.834769