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- Title
Evaluation of the Effect of bFGF Growth Factor on the Ability of Adipose-Derived Stem Cell Differentiation into Bone Lineage.
- Authors
Sheykhhasan, M.; Ghiasi, M.; Tabatabaei-Qomi, R.; Mehdizadeh, M.
- Abstract
Background: In recent years, the extensive research on the potential use of stem cells in the treatment of various diseases, including bone disease has been done, due to the high potential of stem cells. Fat has considered a rich source of stem cells that besides its frequency, easy access, and non-invasive sampling method, has the ability to excellent proliferate and differentiate into other cells. Furthermore, these cells are wonderful and ideal sources for reduction of the immunological incompatibility issues after transplantation. Also, various growth factors, including basic fibroblast growth factor (bFGF), play important roles in the differentiation process of stem cells. Objective: To assess the impact of bFGF growth factor on the differentiation potential of adipose-derived stem cells (ADSCs) into bone lineage. Methods: In this research, stem cells were isolated from the adipose tissue obtained from three patients during nephrectomy. These cells were then placed separately in bone differentiation medium with and without bFGF. The impact of bFGF on the differentiation potential of ADSCs into osteoblast was assessed in 7 and 14 days by real-time PCR. Results: Real-time PCR analysis demonstrated that osteogenic-specific gene expression of cells cultured in the bone differentiation medium containing bFGF had a significant difference compared with cells cultured in the bone differentiation medium without bFGF. Conclusion: Based on our findings, we believed that bFGF has major and effective roles on differentiation of ADSCs into osteogenic lineage.
- Subjects
STEM cells; GROWTH factors
- Publication
International Journal of Organ Transplantation Medicine, 2016, Vol 7, Issue 2, p137
- ISSN
2008-6490
- Publication type
Abstract