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- Title
Reduction in immune cell number and loss of 5hmC are associated with lesion grade in cervical carcinogenesis.
- Authors
Yang, Xiaohan; Shen, Xinyue; Li, Zhujun; Li, Wencai; Liu, Ying
- Abstract
Tumor genome methylation is closely related to tumor immunosuppression. In the present study, we evaluated the fluctuations in DNA methylation levels, and the numbers of infiltrating T cells and their cytokines in different-grade cervical lesions. A total of 154 human cervical specimens that included LSIL (43 cases), HSIL (48 cases), and cervical squamous cancer (63 cases) were used for this study. Immunohistochemistry for 5-hydroxymethylcytosine (5hmC) and T-cell-attracting chemokines was performed, and multiplex immunofluorescence labeling was used to identify different T-cell subtypes. We found that the proportions of samples that immunostained weakly or negatively for 5hmC were increased commensurately with elevations in the severity of cervical lesions. The expression of T-cell-attracting chemokines—including CXCL9, CXCL10, and CXCL11—was positively associated with 5hmC levels, and CXCL9 was the cytokine that was most pronounced. With the progression of cervical lesions, the numbers of total T cells, CTL, and NK cells in the cervical tissues all gradually decreased. During the occurrence and development of cervical squamous carcinoma, 5hmC was gradually lost, and immunosuppression occurred in precancerous cervical lesions.
- Subjects
T cells; CYTOKINES; KILLER cells; PRECANCEROUS conditions; IMMUNOFLUORESCENCE; DNA microarrays
- Publication
3 Biotech, 2021, Vol 11, Issue 11, p1
- ISSN
2190-572X
- Publication type
Article
- DOI
10.1007/s13205-021-03028-8