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- Title
LC-MS/MS Method Development for the Discovery and Identification of Amphidinols Produced by Amphidinium.
- Authors
Wellkamp, Marvin; García-Camacho, Francisco; Durán-Riveroll, Lorena M.; Tebben, Jan; Tillmann, Urban; Krock, Bernd
- Abstract
Amphidinols are polyketides produced by dinoflagellates suspected of causing fish kills. Here, we demonstrate a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the identification and quantification of amphidinols (AM). Novel AM were detected by neutral loss (NL) scan and then quantified together with known AM by selection reaction monitoring (SRM). With the new method, AM were detected in four of eight analyzed strains with a maximum of 3680 fg toxin content per cell. In total, sixteen novel AM were detected by NL scan and characterized via their fragmentation patterns. Of these, two substances are glycosylated forms. This is the first detection of glycosylated AM.
- Subjects
LIQUID chromatography-mass spectrometry; FISH kills
- Publication
Marine Drugs, 2020, Vol 18, Issue 10, p497
- ISSN
1660-3397
- Publication type
Article
- DOI
10.3390/md18100497