We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
The Neuroprotective Marine Compound Psammaplysene A Binds the RNA-Binding Protein HNRNPK.
- Authors
Boccitto, Marco; Lee, Nayoung; Sakamoto, Satoshi; Spruce, Lynn A.; Handa, Hiroshi; Clardy, Jon; Seeholzer, Steven H.; Kalb, Robert G.
- Abstract
In previous work, we characterized the strong neuroprotective properties of the marine compound Psammaplysene A (PA) in in vitro and in vivo models of neurodegeneration. Based on its strong neuroprotective activity, the current work attempts to identify the physical target of PA to gain mechanistic insight into its molecular action. Two distinct methods, used in parallel, to purify protein-binding partners of PA led to the identification of HNRNPK as a direct target of PA. Based on surface plasmon resonance, we find that the binding of PA to HNRNPK is RNA-dependent. These findings suggest a role for HNRNPK-dependent processes in neurodegeneration/neuroprotection, and warrant further study of HNRNPK in this context.
- Subjects
MARINE natural products; NEUROPROTECTIVE agents; RNA-binding proteins; PHENOL derivatives; NUCLEOPROTEINS; SURFACE plasmon resonance
- Publication
Marine Drugs, 2017, Vol 15, Issue 8, p246
- ISSN
1660-3397
- Publication type
Article
- DOI
10.3390/md15080246