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- Title
POLYMORPHISM DETECTION OF IN VITRO CULTIVATED EPILOBIUM (ONAGRACEAE) SPECIES USING RAPD METHOD.
- Authors
Vălimăreanu, Sergiu; Deliu, Constantin
- Abstract
Medicinal plants Epilobium parviflorum Schreb. and Epilobium hirsutum having a phytopharmaceutical importance, used for the treatment of urinary apparatus and prostate diseases, were alternatively cultivated ex vitro/in vitro. By RAPD method, using single primers of arbitrary nucleotide sequence, we attempted to detect genetic polymorphism in purpose of visualizing the putative differences between genetic profiles appeared after in vitro cultivation of the subjected species on culture media supplemented with plant growth regulators, in order to select somaclonal variations. The primers we used were able to detect polymorphisms in absence of specific nucleotide sequence information so that polymorphisms function as genetic and molecular markers.
- Subjects
RESEARCH; MEDICINAL plants; EPILOBIUM; PROSTATE diseases; FERTILIZATION in vitro; GENETIC polymorphisms; POPULATION genetics; NUCLEOTIDE sequence; SEQUENCE alignment
- Publication
Contributii Botanice, 2008, Vol 43, p113
- ISSN
0069-9616
- Publication type
Article