We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Transient Receptor Potential Vanilloid-1 (TRPV1) and Ankyrin-1 (TRPA1) Participate in Visceral Hyperalgesia in Chronic Water Avoidance Stress Rat Model.
- Authors
Yan-Bo Yu; Jing Yang; Xiu-Li Zuo; Li-Jun Gao; Peng Wang; Yan-Qing Li
- Abstract
Stressfull life events have powerful influences on visceral perception of certain IBS patients. In the present study, we aimed to examine the involvement of TRPV1 and TRPA1 in the stress-induced visceral hyperalgesia. Rats were exposed to 1-h water avoidance stress (WAS) daily for 10 consecutive days. The abdominal withdrawal reflex (AWR) to colorectal distension was assessed at the end of the 10-day period. Western-blotting analysis was applied to investigate the alterations of TRPV1 and TRPA1 in the colonic afferent dorsal root ganglia (DRG). Compared with control rats, the WAS-treated rats demonstrated a significant increase in the AWR with the pressure ≥40 mm Hg ( P < 0.05). Meanwhile, in the WAS-treated rats, western-blotting analysis showed significant upregulation of TRPV1 and TRPA1 in the colonic afferent DRG. The results indicate that WAS could induce the upregulation of TRPV1 and TRPA1 in the colonic afferent DRG, and both receptors may be candidate molecules involved in the stress-induced visceral hyperalgesia in rats.
- Subjects
IRRITABLE colon; AFFERENT pathway diseases; ANIMAL models in research; HYPERALGESIA; TRP channels; LABORATORY rats
- Publication
Neurochemical Research, 2010, Vol 35, Issue 5, p797
- ISSN
0364-3190
- Publication type
Article
- DOI
10.1007/s11064-010-0137-z