We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Molecular Characterization of Cry11 Crystal Protein Gene from Bacillus thuringiensis Isolated from Different Soil Samples.
- Authors
Bukhari, Dilara A.; Fatima, Naureen; Rehman, Abdul
- Abstract
The present study was aimed at screening the local Bacilllus thuringiensis (B.t.) isolates for Cry11 and assess their potential use for mosquito control. A total of 15 B.t. isolates were collected, of which 75% were from dry, leaf litter, garden soil samples, 15% were from animal waste and 10% were from moist soil of the crop area. A 650bp of cry11 gene was amplified by PCR and seven isolates were found positive for cry11 gene. The 16S rDNA study exposed that these screened B.t. confirmed 99% homology with B.t. serovar tolworthi, B.t. str. Al Hakam, B.t. serover thuringiensis, B.t. serovar konkukian, B.t. serovar Chinensis. B.t. serover Indiana, and B.t. serover kurstuki. The toxicity bioassays with B.t. spores proved that seven B.t. isolates harboring cry11 gene (viz., NF B.t.,1,2,3,4,5,6,7) were toxic to 3rd instar larvae of mosquito, Aedes aegypti. Among seven B.t. isolates, three isolates NF5 B.t. 7.2, NF1 B.t. 1.1 and NF2 B.t. 4.2 were found most toxic which were isolated from moist soil containing decaying cattle waste, dry waste animal dung and leaf litter soil, respectively. So, these isolates have a great potential to grow into a biopesticidal formulation for control of mosquitoes.
- Subjects
BACILLUS thuringiensis; PROTEIN genetics; SOIL testing; ANIMAL waste; RECOMBINANT DNA
- Publication
Pakistan Journal of Zoology, 2018, Vol 50, Issue 6, p2351
- ISSN
0030-9923
- Publication type
Article
- DOI
10.17582/journal.pjz/2018.50.6.2351.2356