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- Title
Antigen-Specific Proliferative Human T-Lymphocyte Clones with Specificity for Chlamydia trachomatis.
- Authors
Ovigstad, E.; Digranes, S.; Thorsby, E.
- Abstract
Blast-enriched suspensions of T cells primed for Chlamydia trachomatis antigen were cloned by a limiting dilution technique. A cloning efficiency of 20-25% was obtained. T-lymphocyte clones (TLC) with high proliferative responses were selected for further studies. Kinetic studies showed a peak response between 60 and 84 h after antigen stimulation. The TLC were OKT4+. They were specific for the chlamydial antigen and did not respond to other antigens when non-T cells were used as antigen-presenting cells (APC). Antigen-specific proliferation of the TLC required that the responding TLC and the APC shared class-II HLA determinants-that is, HLA-D/DR molecules. The true clonal nature of the TLC was confirmed by subcloning experiments.
- Subjects
T cells; CHLAMYDIA trachomatis; LYMPHOCYTES; CLONE cells; HLA histocompatibility antigens; MOLECULAR cloning
- Publication
Scandinavian Journal of Immunology, 1983, Vol 18, Issue 4, p291
- ISSN
0300-9475
- Publication type
Article