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- Title
Localization of T-Lymphocyte Areas in Human Lymphoid Tissues by Fluorochrome-Labelled Helix pomatia A Haemagglutinin.
- Authors
Zeromski, J.; Hammarström, S.; Hellström, U.; Biberfeld, P.; Perlmann, P.
- Abstract
Fluorochrome-labelled Helix pomatia A haemagglutinin (HP) stained T-dependent areas in cryostat tissue sections of human lymph node, tonsil, and spleen and thymocytes within thymic medulla but not within thymic cortex. Neuraminidase treatment of the tissue section was a prerequisite for the positive cell staining. The staining was cell-membrane associated, and the histological pattern was essentially the same as that observed in parallel sections treated with monoclonal antibodies lo T cells (OKT3, Leu-1). In addition, blood vessel walls and connective tissue were also stained by HP conjugates. The latter staining was independent of neuraminidase treatment, resistant to α-galactosidase treatment and was not blocked by pretreatment with anti-fibronectin antibody. Both types of staining reaction were specific inasmuch as unlabelled HP and the competitive sugar hapten N-acetyl-D-galactosamine blocked the reaction. Frozen, formalin-fixed tissue sections showed the same stainability and reaction pattern as unfixed sections.
- Subjects
LYMPH nodes; HEMAGGLUTININ; LYMPHOID tissue; NEURAMINIDASE; T cells
- Publication
Scandinavian Journal of Immunology, 1982, Vol 16, Issue 2, p83
- ISSN
0300-9475
- Publication type
Article
- DOI
10.1111/j.1365-3083.1982.tb00702.x