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- Title
Differential expression profile of genes encoded in a genome segment of Cotesia plutellae bracovirus in a parasitized host, Plutella xylostella.
- Authors
Gad, Wael; Jae Young Choi; Yeon Ho Je; Yonggyun Kim
- Abstract
The Polydnaviruses are an insect DNA virus group. The segmented genome of a polydnavirus is located on the host wasp chromosome as a provirus. After replication, virions are delivered into the parasitized host, where their expression products play significant roles in specific processes of parasitism. However, little is known about how viral gene expression is controlled. In the present study, we tested whether different genes in a genome segment are expressed concomitantly. Cotesia plutellae bracovirus (CpBV) is a polydnavirus mutualistic to an endoparasitoid, Cotesia plutellae (Braconidae: Hymenoptera). Its circular genome segments in viral particles were captured by a transposon containing pUC origin sequence and replicated in Escherichia coli. CpBV-S30 (23.5 kb), one of the large CpBV genome segments, was fully sequenced, and seven open reading frames (ORF) were predicted. We analyzed expression patterns of the seven ORF in the diamondback moth, Plutella xylostella, parasitized by C. plutellae. Transcription analyses indicated that all ORF of CpBV-S30 in the parasitized P. xylostella were expressed from the first day of parasitization and then expression levels decreased over the period of parasitization, except for CpBV-H4, which showed an additional expression peak. In terms of tissue specificity in their expression patterns, all ORF were found to be expressed in the fat body, hemocytes and gut of the parasitized host. Promoter sequence analysis showed that all seven ORF had typical promoter elements, including TATA binding sites, but promoter component numbers and kinds varied. These results suggest that genes in a genome segment of CpBV can differentially express in the parasitized host, presumably by their different promoter components.
- Subjects
DNA viruses; CHROMOSOMES; VIRAL replication; PARASITISM; GENE expression; ESCHERICHIA coli
- Publication
Entomological Research, 2008, Vol 38, Issue 1, p77
- ISSN
1738-2297
- Publication type
Article
- DOI
10.1111/j.1748-5967.2008.00136.x