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- Title
Prospective evaluation of cell kinetics, yields and donor experiences during a single large-volume apheresis versus two smaller volume consecutive day collections of allogeneic peripheral blood stem cells.
- Authors
Bolan, Charles D.; Carter, Charles S.; Wesley, Robert A.; Yau, Yu Ying; Barrett, A. John; Childs, Richard W.; Read, Elizabeth J.; Leitman, Susan F.
- Abstract
Summary. We report cell kinetics, yields and donation experiences of 20 demographically matched allogeneic peripheral blood stem cell (PBSC) donors who were prospectively assigned to undergo either a single 25 l or two consecutive daily 15 l (15 l × 2) apheresis procedures. Procedures were performed using prophylactic intravenous calcium administration after standard granulocyte colony-stimulating factor (GCSF) mobilization (10 μg/kg/d). Central line placements (two each), initial CD34 cell counts (0·077 vs 0·078 × 109 /l) and yields (7·9 vs 8·1 × 108 CD34 cells) were similar in the two groups; however, 25 l donors spent significantly less time both in the clinic (7·5 vs 10·8 h) and with central venous catheters in place (8·5 vs 29·5 h) than 15 l × 2 donors. End-procedure platelet counts were below 100 × 109 /l in one out of 10 25 l donors versus five out of 10 in 15 l × 2 donors (41%vs 53% mean decrease in platelet counts, P = 0·02). PBSC collection efficiency increased by 37% after 15 l of the 25-l volume had been processed, compared with no significant change during 15 l × 2 procedures. Results similar to these prospective findings were also observed in CD34 yields, symptoms and platelet counts in additional 25 l and 15 l procedures performed during the same period and evaluated retrospectively. This study indicates that a single 25-l apheresis procedure results in similar yields and symptoms, but less donor thrombocytopenia and inconvenience than two consecutive daily 15-l procedures.
- Subjects
STEM cells; HEMAPHERESIS
- Publication
British Journal of Haematology, 2003, Vol 120, Issue 5, p801
- ISSN
0007-1048
- Publication type
Article
- DOI
10.1046/j.1365-2141.2003.04157.x