We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Genetic diversity among Curtobacterium flaccumfaciens pv. flaccumfaciens populations in the American High Plains.
- Authors
Agarkova, I.V.; Lambrecht, P.A.; Vidaver, A.K.; Harveson, R.M.
- Abstract
Curtobacterium flaccumfaciens pv. flaccumfaciens is a Gram-positive bacterium and has reemerged as an incitant of bacterial wilt in common (dry, edible) beans in western Nebraska, eastern Colorado, and southeastern Wyoming. Curtobacterium flaccumfaciens pv. flaccumfaciens is diverse phenotypically and genotypically and is represented by several different pathogen color variants. The population structure of 67 strains collected between 1957 and 2009, including some isolated from alternate hosts, was determined with 3 molecular typing techniques: amplified fragment length polymorphism (AFLP), repetitive extragenic palindromic polymerase chain reaction (rep-PCR), and pulsed-field gel electrophoresis (PFGE). All 3 typing techniques showed a great degree of population heterogeneity, but they were not congruent in cluster analysis of the C. flaccumfaciens pv. flaccumfaciens populations. Cluster analysis of a composite data set (AFLP, PFGE, and rep-PCR) using averages from all experiments yielded 2 distinct groups: cluster A included strains with colonies of yellow, orange, and pink pigments, and cluster B had strains of only yellow pigment. Strains producing purple extracellular pigment were assigned to both clusters. Thus, C. flaccumfaciens pv. flaccumfaciens is diverse phenotypically and genotypically.
- Subjects
BACTERIAL wilt diseases; PATHOGENIC microorganisms; AMPLIFIED fragment length polymorphism; GEL electrophoresis; CLUSTER analysis (Statistics); POLYMERASE chain reaction
- Publication
Canadian Journal of Microbiology, 2012, Vol 58, Issue 6, p788
- ISSN
0008-4166
- Publication type
Article
- DOI
10.1139/w2012-052