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- Title
Application of sequential design for enhanced l-asparaginase synthesis from Ganoderma australe GPC191.
- Authors
Chakraborty, Meghna; Shivakumar, Srividya
- Abstract
With an increasing demand for l-asparaginase in pharmaceutical and food sectors for its cytostatic and acrylamide-reducing qualities, there's a need to discover novel, highly productive enzyme sources with improved pharmacokinetic profiles. Keeping this in mind, the present study aimed at maximizing the potential of Ganoderma australe GPC191 to produce l-asparaginase by fermentation medium optimization using statistical validation. Of the 11 physicochemical parameters evaluated under submerged fermentation conditions through one-factor-at-a-time approach and Plackett–Burman design, only four parameters (inoculum load, l-asparagine, soybean meal, and initial pH) influenced l-asparaginase production, significantly (p < 0.001). The optimal levels and interaction effects of these on the overall production were further evaluated by the central composite rotatable design of response surface methodology. Post-optimization, 27.34 U/mL was predicted as the maximum activity at pH 7 with 5n inoculum load and 15 g/L each of l-asparagine and soybean meal. Experimental validation yielded an activity of 28.52 U/mL, indicating an overall 18.17-fold increase from the unoptimized stage. To our knowledge, this is the first report signifying the l-asparaginase production aptitude of G. australe with sequential statistical validation using agricultural waste, which can serve as a model to enhance its yields, offering a sustainable and cost-effective solution for industrial application.
- Subjects
GANODERMA; SOYBEAN meal; RESPONSE surfaces (Statistics); AGRICULTURAL wastes; FOOD industry; ACRYLAMIDE
- Publication
World Journal of Microbiology & Biotechnology, 2024, Vol 40, Issue 3, p1
- ISSN
0959-3993
- Publication type
Article
- DOI
10.1007/s11274-023-03881-0