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- Title
Production of optically pure 2,3-butanediol from Miscanthus floridulus hydrolysate using engineered Bacillus licheniformis strains.
- Authors
Gao, Yabin; Huang, Huahua; Chen, Shouwen; Qi, Gaofu
- Abstract
2,3-Butanediol (2,3-BD) can be produced by fermentation of natural resources like Miscanthus. Bacillus licheniformis mutants, WX-02ΔbudC and WX-02ΔgldA, were elucidated for the potential to use Miscanthus as a cost-effective biomass to produce optically pure 2,3-BD. Both WX-02ΔbudC and WX-02ΔgldA could efficiently use xylose as well as mixed sugars of glucose and xylose to produce optically pure 2,3-BD. Batch fermentation of M. floridulus hydrolysate could produce 21.6 g/L D-2,3-BD and 23.9 g/L meso-2,3-BD in flask, and 13.8 g/L D-2,3-BD and 13.2 g/L meso-2,3-BD in bioreactor for WX-02ΔbudC and WX-02ΔgldA, respectively. Further fed-batch fermentation of hydrolysate in bioreactor showed both of two strains could produce optically pure 2,3-BD, with 32.2 g/L D-2,3-BD for WX-02ΔbudC and 48.5 g/L meso-2,3-BD for WX-02ΔgldA, respectively. Collectively, WX-02ΔbudC and WX-02ΔgldA can efficiently produce optically pure 2,3-BD with M. floridulus hydrolysate, and these two strains are candidates for industrial production of optical purity of 2,3-BD with M. floridulus hydrolysate.
- Subjects
BUTANEDIOL manufacturing; MISCANTHUS; BACILLUS licheniformis; ENANTIOMERIC purity; XYLOSE
- Publication
World Journal of Microbiology & Biotechnology, 2018, Vol 34, Issue 5, p1
- ISSN
0959-3993
- Publication type
Article
- DOI
10.1007/s11274-018-2450-7