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- Title
Minimally Modified LDL-Induced Impairment of Endothelium-Dependent Relaxation in Small Mesenteric Arteries of Mice.
- Authors
Chen, Gen; Wang, Jun-Jie; Xu, Cang-Bao; Cao, Lei; Lin, Jie; Qing, Xu-Ping; Liu, Si-Yu; Liu, En-Qi; Li, Jie
- Abstract
Minimally modified low-density lipoprotein (mmLDL) is a well-known risk factor for cardiovascular diseases. The present study was designed to investigate the role of mmLDL in the endothelium-dependent relaxation of mouse mesenteric arteries. A sensitive myograph system was employed to examine the endothelial function of mesenteric arteries. mRNA and protein expression levels were determined using real-time PCR and Western blotting, respectively. The ultra-microstructure of mesenteric vascular beds was investigated using a transmission electron microscope. The results showed that mmLDL significantly impaired the acetylcho-line-induced (3 x 10-10 to 1 x 10-4 M) endothelium-dependent relaxation of mouse mesenteric arteries with markedly reduced pIC50 (p < 0.05) and Rmax values (p < 0.001). In addition, mmLDL increased the levels of superoxide production and nitrotyrosine concentration and impaired the endothelial microstructure with decreased KCa3.1 and KCa2.3 expression. In conclusion, mmLDL increases superoxide and nitro-tyrosine levels, damages endothelial microstructure with decreased KCa3.1 and KCa2.3 expression, and ultimately attenuates relaxation mediated by nitric oxide- and endothelium-derived hyperpolarizing factor.
- Subjects
MESENTERIC artery; LOW density lipoproteins; LABORATORY mice; NITRIC oxide; ENDOTHELIUM-derived hyperpolarizing factor
- Publication
Journal of Vascular Research, 2016, Vol 53, Issue 1/2, p58
- ISSN
1018-1172
- Publication type
Article
- DOI
10.1159/000447011