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- Title
Soil bacterial metagenomic analysis from uranium ore deposit of Domiasiat in Northeast India.
- Authors
Kumar, Rakshak; Nongkhlaw, Macmillan; Acharya, Celin; Joshi, Santa Ram
- Abstract
Total bacterial community analyses were performed for uranium ore deposit soil samples of Domiasiat utilizing cultivation-independent approach. Screening based on amplified ribosomal DNA restriction analysis (ARDRA) using MspI and HaeIII was performed to analyse 150 clones which generated 59 distinct ribo-types from the clone library. Representative 96 clone partial 16S rRNA gene were phylogenetically related to 10 different bacterial groups. Proteobacteria and Acidobacteria were the most abundant bacterial group while 7% of the clones represented novel bacterial lineages. The bacterial diversity obtained by the culture-independent approach presented a larger diversity of bacteria as compared to the conditioned cultivation method. The study also provides baseline metagenomic information to assess subsequent impact of environment perturbation consequent to uranium mining at the studied site.
- Subjects
NORTHEASTERN India; SOIL microbiology; METAGENOMICS; URANIUM ore geology; ORE deposits; RIBOSOMAL DNA; PROTEOBACTERIA
- Publication
Current Science (00113891), 2013, Vol 105, Issue 4, p495
- ISSN
0011-3891
- Publication type
Article