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- Title
Identity of differentiation inducing factor and tumour necrosis factor.
- Authors
Takeda, Ken; Iwamoto, Sanju; Sugimoto, Hiroyuki; Takuma, Tetsuo; Kawatani, Norika; Noda, Munehiro; Masaki, Atsushi; Morise, Hiroshi; Arimura, Hirofumi; Konno, Kunio
- Abstract
Human myelogenous leukaemic cells can be induced to differentiate into the monocyte/macrophage pathway by protein inducers called differentiation inducing factors (DIF) in conditioned media of mitogen-stimulated human peripheral blood leukocytes1-8. However, human DIF has not yet been well characterized. DIF is known to be a T-cell lymphokine, as it can be obtained from the T-cell line HUT-1027 and can be partially purified from medium conditioned by phytohaemagglutinin (PHA)-stimulated lymphocytes8. We found that monocytes also produce factor(s) that induce differentiation of human myelogenous leukaemia cell lines to cells with macrophage-like characteristics. This factor(s) has activity different from that of colony-stimulating factor(s) or interferons9 . We have now purified a DIF to homogeneity from medium conditioned by PHA-stimulated leukocytes using a human myeloblastic leukemia cell line, ML-110, as target cells. The purified DIF has a relative molecular mass (Mr) of approximately 17,000, with an NH2-terminal sequence the same as that of human tumour necrosis factor (TNF). Recombinant human TNF (rHuTNF) induces differentiation of ML-1 cells and an anti-pDIF monoclonal antibody can neutralize both differentiation inducing activity and cytotoxic activity of DIF and rHuTNF. The findings indicate that one of the DIF(s) produced by leukocytes is probably TNF.
- Publication
Nature, 1986, Vol 323, Issue 6086, p338
- ISSN
0028-0836
- Publication type
Article
- DOI
10.1038/323338a0