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- Title
Spray-dried extract of P hyllanthus niruri L . reduces mucosal damage in rats with intestinal inflammation.
- Authors
Melo, Maryanne Nunes de; Soares, Luiz Alberto Lira; Porto, Cynthia Raquel da Costa; Araújo, Aurigena Antunes de; Almeida, Maria das Graças; Souza, Tatiane Pereira de; Petrovick, Pedro Ros; Araújo, Raimundo Fernandes de; Guerra, Gerlane Coelho Bernardo
- Abstract
P hyllanthus niruri L. belongs to the Euphorbiaceae, and is known by the common name of 'stonebreaker' in Brazil. Some species within the P hyllanthus genus are widely used in traditional medicine to counteract different types of anti-inflammatory diseases. Objectives In this study, the preventive intestinal anti-inflammatory activity of spray-dried extract of P . niruri ( SDEPn) was tested in the model of acetic acid (10%)-induced ulcerative colitis in the rat. Methods Colitis animals were given orally at doses 25, 100 and 200 mg/kg. Colons tissue was analysed by macroscopic score, by histopathology score, by the immunohistochemical examination of tumour necrosis factor alpha, p53 and interferon gamma; by spectroscopic ultraviolet-visible spectrophotometry ( UV/ VIS) analysis of the levels of myeloperoxidase, malonaldehyde and total glutathione. Key Findings/Result Pretreatment of the extract to colitic rats significantly attenuated colonic macroscopic damage induced by acetic acid ( P < 0.01). Spray-dried extract of P . niruri prevented glutathione depletion ( P < 0.001) and malondialdehyde levels ( P < 0.05) declined. Spray-dried extract of P . niruri significantly reduced microscopic damage to tissues, such as leukocyte infiltration accompanied by a significant reduction in myeloperoxidase activity ( P < 0.5). Immunohistochemistry revealed a decline in the TNF-α, IFN-γ and p53 protein ( P < 0.05). Conclusion Spray-dried extract of P . niruri has a beneficial effect in the acute phase of acetic acid-induced colitis in the rat, which is probably related to its antioxidant properties.
- Subjects
MUCOUS membranes; PLANT extracts; PHYLLANTHUS; INFLAMMATION treatment; TRADITIONAL medicine; LABORATORY rats; WOUNDS &; injuries
- Publication
Journal of Pharmacy & Pharmacology, 2015, Vol 67, Issue 8, p1107
- ISSN
0022-3573
- Publication type
Article
- DOI
10.1111/jphp.12408