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- Title
Obtainment and Characterization of Alhagi persarum Boiss. et Buhse Callus Cell Cultures that Produce Isoflavonoids.
- Authors
Titova, M. V.; Kochkin, D. V.; Sobolkova, G. I.; Fomenkov, A. A.; Sidorov, R. A.; Nosov, A. M.
- Abstract
The physiological characteristics of callus cell cultures of Alhagi persarum Boiss et Buhse, a representative of the legume family widely used in folk medicine, have been studied. It was shown that the explant source is an important factor influencing the efficiency of callus initiation: more intense callus formation (almost 100%) was observed for explants excised from various organs of sterile seedlings as compared to intact plants (less than 30%). As a result, more than 20 lines of morphologically different callus cultures were obtained, and the growth parameters were determined for the five lines with the most intensive growth. The composition of fatty acids (FAs) of total lipids and secondary metabolites in the most physiologically stable callus line, Ar-207, was analyzed. Nineteen individual C12–C24 FAs were identified via capillary gas-liquid chromatography with mass spectrometric detection; the majority of them were palmitic (~23%), stearic (~22%), linoleic (~14%), or α-linolenic (~33%) acids. The established atypical composition of FAs (a simultaneous high content of both saturated FAs and polyunsaturated α-linolenic acid) is likely due to the adaptation of cells to in vitro growth conditions. Phytochemical analysis of secondary metabolites was carried out with ultra-performance liquid chromatography and mass spectrometric detection via electrospray ionization. Compounds belonging to different structural groups of isoflavones were found. Aglycones (calycosin, formononetin and afrormosin isomer), glycosides (formononetin glucoside), and esters of glucosides (malonylglycosides of calycosin, formononetin, afrormosin isomers, glycitein, and genistein) were detected. These secondary metabolites are widespread in plants of the Fabaceae family; however, isoflavones are rare in representatives of the Alhagi genus. The presence of malonylated isoflavone glycosides in Alhagi ssp. was shown for the first time.
- Subjects
CELL culture; ISOFLAVONES; CALLUS; ISOFLAVONOIDS; GAS chromatography; METABOLITES; CELL suspensions; CALLUS (Botany)
- Publication
Applied Biochemistry & Microbiology, 2021, Vol 57, Issue 8, p866
- ISSN
0003-6838
- Publication type
Article
- DOI
10.1134/S000368382108007X