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- Title
Establishment of a loop-mediated isothermal amplification (LAMP) detection method for genetically modified maize MON88017.
- Authors
Zhen, Zhen; Zhang, Minghui; Yu, Yanbo; Gao, Xuejun; Zhu, Yanming; Yan, Yu; Zhang, Renren
- Abstract
In this study, we developed a visual and rapid assay for the detection of MON88017 maize using the LAMP method. The LAMP method was specific for MON88017 event and takes only 40 min and the LAMP assay sensitivity is about 40 copies, which is the same level as that of conventional PCR method. LAMP amplicons can directly be detected by naked-eye inspection after adding SYBR Green I. In summary, the LAMP method is visual, faster, and more sensitive and does not need special equipment compared to the traditional PCR technique, which makes it a very higher efficiency approach for field tests and fast screening of GMO crops, especially for on-site, large-scale testing purposes in the field.
- Subjects
TRANSGENIC plants; CORN analysis; GENE amplification; POLYMERASE chain reaction; FOOD research
- Publication
European Food Research & Technology, 2016, Vol 242, Issue 10, p1787
- ISSN
1438-2377
- Publication type
Article
- DOI
10.1007/s00217-016-2678-0