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- Title
康复新在巨噬细胞炎症反应中的抗炎作用及其机.
- Authors
汤雁利; 张玉皓; 李罡; 李启
- Abstract
Objective To observe the anti-inflammatory effects of Kangfuxin in cell inflammation of macrophages and to discuss the possible mechanism. Methods The cultivated mouse macrophages RAW264. 7 were randomly divided into 6 groups:group A (added with DMSO),group B (added with DMSO),group C (added with anti-inflammation drug BAY11-7082),group D (added with 1% Kangfuxin),group E (added with 0. 1% Kangfuxin),and group F (added with 0. 01% Kangfuxin). We continued to cultivate for 1 h after drug intervention,group A was treaded with DMEM,the other groups were treated with LPS (100 μg/ L)to induce cell inflammation. The cells were collected after treatment of 4 h and 8 h,the mRNA expression levels of IL-6,TNF-α and prostaglandin-endoperoxide synthase (PTGS2)were detected by qRT-PCR. Results Four hours and eight hours after modeling,compared with group A,the mRNA expression levels of IL-6,TNF-α and PTGS2 were increased in the group B (all P < 0. 05);compared with group B,the mRNA expression levels of IL-6,TNF-α and PTGS2 were decreased in the group C (all P <0. 05). All cells were dead in the group D. Compared with group B,the mRNA expression of IL-6 and TNF-α was increased and the PTGS2 mRNA expression was decreased in the groups E and F (all P <0. 05). Compared with group C,significant difference was found in the mRNA expression of IL-6 and TNF-α between groups E and F (all P <0. 05),and no significant difference was found in the mRNA expression of PTGS2 between groups E and F (all P >0. 05). The concentration and duration of Kangfuxin had no effect on the mRNA expression of IL-6,TNF-α and PTGS2. Conclusion Kangfuxin has inhibitory effect on the cell inflammatory reaction,and its mechanism may be not related with the inhibition of expression of inflammatory factors,such as IL-6 and TNF-α,but be related with the down-regulated PTGS2 expression.
- Publication
Shandong Medical Journal, 2016, Vol 56, Issue 9, p13
- ISSN
1002-266X
- Publication type
Article
- DOI
10.3969/j.issn.1002-266X.2016.09.005