We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
MeGLYI-13, a Glyoxalase I Gene in Cassava, Enhances the Tolerance of Yeast and Arabidopsis to Zinc and Copper Stresses.
- Authors
Li, Ruimei; Tang, Fenlian; Che, Yannian; Fernie, Alisdair R.; Zhou, Qin; Ding, Zhongping; Yao, Yuan; Liu, Jiao; Wang, Yajie; Hu, Xinwen; Guo, Jianchun
- Abstract
Although zinc and copper are the two essential nutrients necessary for plant growth, their excessive accumulation in soil not only causes environmental pollution but also seriously threatens human health and inhibits plant growth. The breeding of plants with novel zinc or copper toxicity tolerance capacities represents one strategy to address this problem. Glyoxalase I (GLYI) family genes have previously been suggested to be involved in the resistance to a wide range of abiotic stresses, including those invoked by heavy metals. Here, a MeGLYI-13 gene cloned from a cassava SC8 cultivar was characterized with regard to its potential ability in resistance to zinc or copper stresses. Sequence alignment indicated that MeGLYI-13 exhibits sequence differences between genotypes. Transient expression analysis revealed the nuclear localization of MeGLYI-13. A nuclear localization signal (NLS) was found in its C-terminal region. There are 12 Zn2+ binding sites and 14 Cu2+ binding sites predicted by the MIB tool, of which six binding sites were shared by Zn2+ and Cu2+. The overexpression of MeGLYI-13 enhanced both the zinc and copper toxicity tolerances of transformed yeast cells and Arabidopsis seedlings. Taken together, our study shows the ability of the MeGLYI-13 gene to resist zinc and copper toxicity, which provides genetic resources for the future breeding of plants resistant to zinc and copper and potentially other heavy metals.
- Subjects
CASSAVA; GLYOXALASE; ZINC; PLANT breeding; COPPER; COPPER poisoning; HEAVY-metal tolerant plants
- Publication
Plants (2223-7747), 2023, Vol 12, Issue 19, p3375
- ISSN
2223-7747
- Publication type
Article
- DOI
10.3390/plants12193375