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- Title
野生种西印度瓜与甜瓜种间杂种的创制.
- Authors
郑莉娜; 毕研胜; 孙亚亭; 王克征; 钱春桃; 李季; 程春燕; 娄群峰
- Abstract
[Objectives]The purpose of this article was to improve the cultivation of melon by using foreign excellent exogenous genes to broaden its genetic basis. [Methods]12 cultivated species of common melon and 1 wild species of Cucumis anguria L. were used as reciprocal cross parents, and the F1 hybrids with C.anguria as female parent and cultivated melon ’Xinhongrui’as male parent were obtained by using the immature embryo culture technique. And the F1 was backcrossed with melon to obtain BC1 plants. The results of F1 and BC1 were evaluated by morphological, cytological and molecular analysis. [Results]In the process of embryo rescue, three of the four young embryos germinated successfully. Through subculture and domestication, four hybrid offspring plants were finally preserved. The results showed that the F1 was yellowing and grew slowly, the leaf shape and the male flower size of the F1 were intermediate to those of either parent in the early stage. But these traits were more inclined to female parents in the later stage, and the botanical characteristics of male parents disappeared. The two characters of fruit stalk length and stem villi always showed intermediate type. SSR identification showed that both parental bands and some new bands were amplified in F1. The authenticity of interspecific hybrid F1 was verified by morphology and SSR molecular marker identification. There were two mature seeds of BC1 which were obtained by backcrossing F1 with melon parent. BC1 plants were identified as bands of C.anguria in molecular markers. In the field investigation, it was found that the tendrils of BC1 had bitter taste, while the tendrils of C.anguria and ’Xinhongrui’had no bitter taste. [Conclusions]The real interspecific hybrid F1 was obtained, and it could lead to the preliminary transduction of exogenous genetic materials into cultivated melon, which provided a theoretical basis and technical reference for improving cultivated melon by using the excellent genes of C.anguria.
- Subjects
BITTERNESS (Taste); WATERMELONS; GENETIC transduction; MELONS; EMBRYOS
- Publication
Journal of Nanjing Agricultural University / Nanjuing Nongye Daxue Xuebao, 2023, Vol 46, Issue 3, p464
- ISSN
1000-2030
- Publication type
Article
- DOI
10.7685/jnau.202204010