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- Title
Identification of Genes Induced in Chondrocytes by Nitric Oxide.
- Authors
Nikolaev, A. I.; Dubovaya, V. I.; Litvinov, D. Yu.; Poltaraus, A. B.; Ivanov, D. S.; Amchenkova, A. M.; Narovlyanskii, A. N.; Panasyuk, A. F.; Prasolov, V. S.; Turpaev, K. T.
- Abstract
Nitric oxide (NO) acts as a short-lived paracrine factor and selectively activates transcription of certain genes. The spectrum of inducible genes was studied in primary chondrocytes. A cDNA library was obtained by subtraction hybridization with RNAs isolated from rabbit chondrocytes before and after treatment with nitrosoglutathione, an NO-generating agent. Some of the cloned cDNAs were homologous to known mammalian genes and human EST. NO-dependent transcriptional activation was demonstrated for the stromelysin 1 and cyclooxygenase 2 genes and, for the first time, for mcl1 coding for an apoptosis suppressor.
- Subjects
GENES; CARTILAGE cells; NITRIC oxide; CELL death; CYCLOOXYGENASES; MOLECULAR genetics
- Publication
Molecular Biology, 2002, Vol 36, Issue 5, p671
- ISSN
0026-8933
- Publication type
Article
- DOI
10.1023/A:1020623413931