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- Title
Cell surface expression and internalization of the murine erythroid AE1 anion exchanger tagged with an extracellular FLAG epitope.
- Authors
Wang, Chen-Chi; Sato, Kota; Otsuka, Yayoi; Inaba, Mutsumi
- Abstract
The article presents a study which analyzes internalization and cell surface expression of mutants of murine erythroid anion exchanger 1 (AE1) in a membrane skeletal network, which is tagged with an extracellular FLAG epitope. The study investigates the expression of the AE1 mutants through methods such as biotinylation, deglycosylation, and immunofluorescence microscopy. The results show that cell surface expression of AE1 does not involved the processing of N-glycan as a prerequisite process.
- Subjects
MEMBRANE proteins; ERYTHROCYTE membranes; EPITOPES; GENETIC mutation; IMMUNOFLUORESCENCE; GLYCANS
- Publication
Japanese Journal of Veterinary Research, 2011, Vol 59, Issue 4, p157
- ISSN
0047-1917
- Publication type
Article