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- Title
Effect of calcium-activated potassium channels on EDHF-mediated endothelial diastolic dysfunciion induced by high glucose.
- Authors
ZHAO Li-mei; WANG Yan; YANG Yong; YANG Yu-bai; DENG Xiu-ling
- Abstract
Objective To investigate the role of endothelial calcium-activated potassium channels in endothelium-derived hyperpolarizing factor (EDHF)-mediated endothelial diastolic dysfunction induced by high glucose. Methods The changes of ACh-induced vasodilatation mediated by IKCa and SKCa in mesentery arterioles of rats incubated with high glucose (mannitol as control) for 3 hours were measured by multi-myograph system. The protein levels of KCa3.1 and KCa2.3 in cultured human umbilical vein endothelial cells (HUVECs) were detected by Western blot. Results The vasodilatation mediated by EDHF, IKCa and SKCa was impaired in 30mmol/L glucose-incubated mesentery arterioles, but not in 30mmol/L mannitol-incubated ones. Incubation with 30mmol/L glucose for 48 hours significantly decreased the protein expression of KCa3.1 and KCa2.3. Conclusion High glucose impairs EDHF-mediated relaxation, which may be related to the down-regulation of KCa channels.
- Subjects
ENDOTHELIUM-derived hyperpolarizing factor; ENDOTHELIAL cells; VASODILATION; WESTERN immunoblotting; UMBILICAL veins; MANNITOL
- Publication
Journal of Xi'an Jiaotong University (Medical Sciences), 2013, Vol 34, Issue 5, p610
- ISSN
1671-8259
- Publication type
Article
- DOI
10.7652/jdyxb201305012