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- Title
Evaluations of Phytochemical Contents, Antibacterial and Antioxidant Potential of Methanolic Leaves Extract of Tanacetum camphoratum Less. (Camphor tansy).
- Authors
Khan, Muhammad Ajmal; Yahya, Muhammad; Hazrat, Ali; Khan, Javed; Jan, Saeed; Nowsheen, Tabinda; Ullah, Inam
- Abstract
Tanacetum camphoratum is a rhizomatous perennial herb. The current study investigated the phytochemical constituents, antioxidants, and antibacterial activities of methanolic extracts of T. camphoratum leaves. The methanolic extract was quantitatively evaluated for alkaloids, flavonoids and terpenoids contents. The antibacterial activity of methanolic leaves extract of T. camphoratum was carried out against Gram-positive and Gram-negative bacterial strains through agar well diffusion assay (small wells were made with cork borer and 6µl of plant extract was added to the wells). The antioxidant activity of methanolic leaves extract was evaluated through a well established protocol 2,2-diphenyl-picrylhydrazyl hydrate (DPPH) at various concentrations (50, 100, 150, 200, and 250 µL). The phytochemical analysis of methanolic leaves extract of T. camphoratum showed 2.80% alkaloids, 4.90% flavonoids and 1.80% terpenoids content. The antibacterial activity of the methanolic extract showed high effectiveness against Gram-positive bacteria (MRSA) with 23 mm zone of inhibition (ZOI), whereas against Gram-negative bacteria, S. typhi, E. coli and P. aeruginosa, with ZOI observed was 22, 18 and 20 mm, respectively. Antioxidant activity evaluated by using different concentrations of methanolic extracts showed good activity at 250 µL through DPPH, which depicted the vibrancy in phenolic, flavonoids and terpenoids contents. This insightful study highlighted the potential usage of methanolic leaves extract of T. camphoratum as a broad-spectrum antibacterial agent and an antioxidant source in food and pharmaceutical industry.
- Subjects
PHYTOCHEMICALS; ESCHERICHIA coli; GRAM-positive bacteria; GRAM-negative bacteria; PLANT extracts; EXTRACTS
- Publication
Sarhad Journal of Agriculture, 2022, Vol 38, Issue 3, p989
- ISSN
1016-4383
- Publication type
Article
- DOI
10.17582/journal.sja/2022/38.3.989.996