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- Title
Study on Quality Standard of Tibetan Medicine Corydalis dasyptera Maxim.
- Authors
Yi SHI; Yunfeng HAN; Xi TANG; Jie GAO; Lin PAN; Yi ZHANG; Xianrong LAI; Duojie LONGZHU
- Abstract
[Objectives] To establish the quality standard of Tibetan medicine Corydalis dasyptera Maxim. [Methods] According to the research method of drug quality standard in the appendix of 2020 edition of Chinese Pharmacopoeia, 8 batches of C. dasyptera Maxim. from different habitats were studied by character identification, microscopic identification and TLC identification. The content of water, total ash, acid-insoluble ash and alcohol-soluble extract was determined, and the content of protopine in medicinal materials was determined by high performance liquid chromatography(HPLC). [Results] The properties and microscopic characteristics of C. dasyptera Maxim. were determined. The TLC characteristic spots of the medicinal materials were clear, the degree of separation was good, and the specificity was strong. Both the test sample and the control sample showed the same yellow-green spots in the corresponding position. It was tentatively determined that the water content of C. dasyptera Maxim. should not exceed 14.0%, the total ash content should not exceed 14.0%, the acid-insoluble ash content should not exceed 3.0%, and the alcohol-soluble extract content should not be less than 18.0%. There was a good linear relationship between the concentration of protopine and the peak area in the range of 16.64-166.40 μg ·10~(-3)(r=0.999 6). The average recovery rate was 98.47% and the RSD was 1.21%(n=6). The content of protopine in 8 batches of C. dasyptera Maxim. was 0.023%-0.093%. [Conclusions] The established quality research method is simple, stable and reliable, and can be used for the quality control of C. dasyptera Maxim.
- Subjects
TIBETAN medicine; QUALITY standards; CORYDALIS; HIGH performance liquid chromatography; QUALITY control
- Publication
Medicinal Plant, 2021, Vol 12, Issue 5, p28
- ISSN
2152-3924
- Publication type
Article
- DOI
10.19600/j.cnki.issn2152-3924.2021.05.007