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- Title
Selective in vivo transfection of murine biliary epithelia using polycation-enhanced adenovirus.
- Authors
McKay, T R; MacVinish, L J; Carpenter, B; Themis, M; Jezzard, S; Goldin, R; Pavirani, A; Hickman, M E; Cuthbert, A W; Coutelle, C
- Abstract
We have investigated the use of polycations to increase adenovirus-mediated expression of transgenic protein to the biliary epithelia with a view to gene therapy for hepatobiliary disease in CF. We have shown that adenovirus carrying the β-galactosidase transgene transfect both human and mouse biliary epithelia in primary culture and that in both instances adenovirus transfection can be significantly increased by co- complexing with polycation. In vivo administration of 1 × 10[sup 9] p.f.u. adenovirus co-complexed with the polyamine polyethy- enimine (PEI) into the mouse biliary duct leads to >80% positively stained biliary epithelia while adenovirus alone at the same titre infected <5% biliary epithelia. We suggest that the use of low titre polycation enhanced adenoviral delivery to the biliary tree of CF patients could be of therapeutic significance. As a prelude to an extensive in vivo functional investigation in CF null mice we have shown that Ad5/polycation complexes deliver functional CFTR to nonCFTR expressing cells in vitro more efficiently than AdS alone.
- Subjects
ADENOVIRUSES; EPITHELIUM; GENE therapy
- Publication
Gene Therapy, 2000, Vol 7, Issue 8, p644
- ISSN
0969-7128
- Publication type
Article
- DOI
10.1038/sj.gt.3301144