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- Title
1,25-Dihydroxyvitamin D3 Deficiency is Involved in the Pathogenesis of Diabetic Retinopathy in the Uygur Population of China.
- Authors
Yi, Xianglong; Sun, Jialin; Li, Li; Wei, Qin; Qian, Yi; Chen, Xueyi; Ma, Ling
- Abstract
1,25-Dihydroxyvitamin D3 [1,25(OH)2D3] has recently been shown to have immunomodulatory property. This study aimed to investigate the expression and potential role of 1,25(OH)2D3 in the pathogenesis of diabetic retinopathy (DR) in the Uygur population. Blood samples were obtained from 22 patients with proliferative DR (PDR), 29 patients with nonproliferative DR (NPDR), and 24 normal controls. ELISA was performed to estimate the serum levels of 1,25(OH)2D3. Peripheral blood mononuclear cells (PBMCs) were cultured with or without 1,25(OH)2D3 in the presence of anti-CD3 and anti-CD28 antibodies to detect the secretion of cytokines and cell proliferation. The FACS cytometric bead array system was used to analyze cytokine levels in the serum and culture supernatants. The Cell Counting Kit was used to determine the rate of cell proliferation. In this study, we found that the patients with PDR showed a decreased serum level of 1,25(OH)2D3 and increased production of IFN-γ, TNF-α, IL-6, and IL-17A, by anti-CD3 and anti-CD28 antibodies activated PBMCs. Furthermore, 1,25(OH)2D3 significantly inhibited the proliferation of PBMCs, as well as the secretion of IFN-γ, TNF-α, IL-6, and IL-17A. Overall, our findings suggest a potential protective effect of 1,25(OH)2D3 in DR, whereas supplementation with 1,25(OH)2D3 might be an effective strategy for preventing the development of DR. © 2016 IUBMB Life, 68(6):445-451, 2016
- Subjects
CHINA; CHOLECALCIFEROL; VITAMIN deficiency; DIABETIC retinopathy; PUBLIC health; IMMUNOMODULATORS
- Publication
IUBMB Life, 2016, Vol 68, Issue 6, p445
- ISSN
1521-6543
- Publication type
Article
- DOI
10.1002/iub.1501