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- Title
Pectobacterium atrosepticum Phage vB_PatP_CB5: A Member of the Proposed Genus ‘Phimunavirus’.
- Authors
Buttimer, Colin; Lucid, Alan; O’Mahony, Jim; Coffey, Aidan; Neve, Horst; Franz, Charles M. A. P.; Turner, Dann; Lavigne, Rob
- Abstract
<italic>Pectobacterium atrosepticum</italic> is a phytopathogen of economic importance as it is the causative agent of potato blackleg and soft rot. Here we describe the <italic>Pectobacterium</italic> phage vB_PatP_CB5 (abbreviated as CB5), which specifically infects the bacterium. The bacteriophage is characterized in detail and TEM micrographs indicate that it belongs to the <italic>Podoviridae</italic> family<italic>.</italic> CB5 shares significant pairwise nucleotide identity (≥80%) with <italic>P. atrosepticum</italic> phages φM1, Peat1, and PP90 and also shares common genome organization. Phylograms constructed using conserved proteins and whole-genome comparison-based amino acid sequences show that these phages form a distinct clade within the <italic>Autographivirinae</italic>. They also possess conserved RNA polymerase recognition and specificity loop sequences. Their lysis cassette resembles that of <italic>KP34virus</italic>, containing in sequential order a U-spanin, a holin, and a signal–arrest–release (SAR) endolysin. However, they share low pairwise nucleotide identity with the type phage of the <italic>KP34virus</italic> genus, <italic>Klebsiella</italic> phage KP34. In addition, phage KP34 does not possess several conserved proteins associated with these <italic>P. atrosepticum</italic> phages. As such, we propose the allocation of phages CB5, Peat1, φM1, and PP90 to a separate new genus designated <italic>Phimunavirus</italic>.
- Subjects
ERWINIA; PHYTOPATHOGENIC microorganisms; BACTERIOPHAGES; PODOVIRIDAE; GENOMES
- Publication
Viruses (1999-4915), 2018, Vol 10, Issue 8, p394
- ISSN
1999-4915
- Publication type
Article
- DOI
10.3390/v10080394