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- Title
In vitro Propagation for Rapid and Efficient Regeneration of Cardiospermum halicacabum L. – An important Medicinal Plant.
- Authors
Kumar, Ashwani; Goyal, S. C.; Pooja; Parshad, Jagdish; Singh, Anshuman; Sheoran, Parvender; Lata, Charu; Rajkumar; Jain, Meenakshi
- Abstract
Cardiospermum halicacabum, a medicinial plant rich in β-sitosterol, Dglucoside, saponin and quebrachitol. Direct regeneration was achieved only from the nodal explants which showed 100 per cent sprouting on MS medium supplemented with BAP (0.5 ppm) + NAA (1.0 ppm) in 12 days, while MS medium supplemented with BAP (0.5 ppm) + IBA (1, 2 or 3 ppm) showed early but decreased sprouting. The half strength MS basal medium without any growth regulator proved to be best medium for root induction for excised shoots. The in vitro raised plantlets were transplanted to mixture of sterile dune sand and FYM (3:1) with moderate (80 %) success.
- Subjects
PLANT micropropagation; REGENERATION (Botany); MEDICINAL plants; SAPONINS; GERMINATION; PLANT regulators
- Publication
Current Trends in Biotechnology & Pharmacy, 2016, Vol 10, Issue 4, p368
- ISSN
0973-8916
- Publication type
Article