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- Title
Antibiotic resistance profiles and activity of clove essential oil (Syzygium aromaticum) against Pseudomonas aeruginosa isolated of canine otitis.
- Authors
Vieira Costa, Larissa; Assunção Rosa Moreira, Janaina Marcela; de Godoy Menezes, Isabela; Dutra, Valéria; Parto Ferreira de Almeida, Arleana do Bom
- Abstract
Background and Aim: Pseudomonas aeruginosa is often isolated from acute and chronic otitis and deep pyoderma in dogs. The increase in bacterial resistance to antibiotics induced the need for alternative therapies to treat infections, with an emphasis on essential oils (EOs). This study aimed to investigate clove oil's in vitro bactericidal action as a therapeutic alternative against strains of P. aeruginosa isolated from canine otitis. Materials and Methods: The antibacterial activity of clove oil was evaluated by determining the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) using the broth microdilution technique in 96-well plates. Serial concentrations of 10-0.31% of the oil were used, equivalent to 104.5-3.26 mg/mL. The susceptibility of isolates to different classes of antibiotics was determined by the disk diffusion technique using 20 antibiotics belonging to eight classes. Isolates resistant to at least one antibiotic of three different classes were considered multidrug-resistant (MDR). Results: A high occurrence of resistance was observed for three antibiotics belonging to the cephalosporin classes (cefadroxil, cephalexin, and ceftriaxone), namely, sulfamethoxazole + trimethoprime, doxycycline, and enrofloxacin. The lowest resistance rates were observed for meropenem (4.88%), amikacin (12.20%), and tobramycin (12.2%). All isolates were susceptible to clove oil with an equivalent MIC and MBC from 3.26 to 6.53 mg/mL. Eugenol was the major component of the oil. Conclusion: Clove EO was effective against MDR strains of P. aeruginosa, indicating an alternative for developing an efficient and low-cost antimicrobial agent to treat canine otitis.
- Subjects
BACTERICIDAL action; ESSENTIAL oils; AMIKACIN; CLOVE tree; PSEUDOMONAS aeruginosa; DRUG resistance in bacteria; OTITIS; ANTIBACTERIAL agents
- Publication
Veterinary World, 2022, Vol 15, Issue 10, p2499
- ISSN
0972-8988
- Publication type
Article
- DOI
10.14202/vetworld.2022.2499-2505