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- Title
Detection of Mycobacterium tuberculosis and Mycobacterium bovis in Sahiwal cattle from an organized farm using ante-mortem techniques.
- Authors
Filia, Gursimran; Leishangthem, Geeta Devi; Mahajan, Vishal; Singh, Amarjit
- Abstract
Aim: The aim of this study was to investigate the prevalence of bovine tuberculosis (TB) and detection of Mycobacterium bovis in cattle from an organized dairy farm. Materials and Methods: A total of 121 animals (93 females and 28 males) of 1 year and above were studied for the prevalence of bovine TB using single intradermal comparative cervical tuberculin (SICCT) test, bovine gamma-interferon (γ-IFN) enzyme immunoassay, and polymerase chain reactions (PCRs). Results: Out of total 121 animals, 17 (14.04%) animals were positive reactors to SICCT test while only one (0.82%) animal for γ-IFN assay. By PCR, Mycobacterium TB complex was detected in 19 (15.70%) animals out of which 4 (3.30%) animal were also positive for M. bovis. Conclusions: Diagnosis of bovine TB can be done in early stage in live animals with multiple approaches like skin test followed by a molecular technique like PCR which showed promising results.
- Subjects
MYCOBACTERIUM bovis; MYCOBACTERIUM avium paratuberculosis; DAIRY farm management; ENZYME-linked immunosorbent assay; POLYMERASE chain reaction; DIAGNOSIS
- Publication
Veterinary World, 2016, Vol 9, Issue 4, p383
- ISSN
0972-8988
- Publication type
Article
- DOI
10.14202/vetworld.2016.383-387