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- Title
Avian Influenza Viruses Infect Primary Human Bronchial Epithelial Cells Unconstrained by Sialic Acid &agr;2,3 Residues.
- Authors
Oshansky, Christine M.; Pickens, Jennifer A.; Bradley, Konrad C.; Jones, Les P.; Saavedra-Ebner, Geraldine M.; Barber, James P.; Crabtree, Jackelyn M.; Steinhauer, David A.; Tompkins, S. Mark; Tripp, Ralph A.
- Abstract
Avian influenza viruses (AIV) are an important emerging threat to public health. It is thought that sialic acid (sia) receptors are barriers in cross-species transmission where the binding preferences of AIV and human influenza viruses are sias &agr;2,3 versus &agr;2,6, respectively. In this study, we show that a normal fully differentiated, primary human bronchial epithelial cell model is readily infected by low pathogenic H5N1, H5N2 and H5N3 AIV, which primarily bind to sia &agr;2,3 moieties, and replicate in these cells independent of specific sias on the cell surface. NHBE cells treated with neuraminidase prior to infection are infected by AIV despite removal of sia &agr;2,3 moieties. Following AIV infection, higher levels of IP-10 and RANTES are secreted compared to human influenza virus infection, indicating differential chemokine expression patterns, a feature that may contribute to differences in disease pathogenesis between avian and human influenza virus infections in humans.
- Subjects
INFLUENZA A virus; BRONCHIAL blood vessels; SIALIC acids; EPITHELIAL cells; PUBLIC health; INFLUENZA viruses; CROSS-species amplification; CHEMOKINES; MOIETIES (Chemistry); DISEASES
- Publication
PLoS ONE, 2011, Vol 6, Issue 6, p1
- ISSN
1932-6203
- Publication type
Article
- DOI
10.1371/journal.pone.0021183