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- Title
SYSTEMIC ANTIVIRAL RESISTANCE INDUCED IN PAPAYA BY CAP-34, A RESISTANCE INDUCING PROTEIN FROM CLERODENDRUM ACULEATUM, IS ASSOCIATED WITH A PROTEINACEOUS VIRUS INHIBITORY ACTIVITY.
- Authors
Srivastava, A.; Srivastava, S.; Prasad, V.
- Abstract
Proteins from non-host higher plants are known to induce systemic resistance in plants against virus infections in susceptible plants. CAP-34 is one such known protein with molecular mass 34 kDa, isolated from Clerodendrum aculeatum. CAP-34 was also shown to be acting against Papaya ringspot virus (PRSV) in papaya by imposing a possible check on PRSV replication. Since the induced resistance was systemic, efforts were made to identify host components that could be involved in it. In the process, a virus inhibitory activity was observed concomitantly with the development of resistance. This paper reports the time-course of virus inhibitory activity, purification to electrophoretic homogeneity of a proteinaceous virus inhibitory agent (VIA), and the determination of some of its biochemical characteristics. Called CP-VIA-34, on the basis of the host it was isolated from (Carica papaya), and the determined molecular mass (34 kDa), this VIA was found to be a basic protein that inactivated Tobacco mosaic virus (TMV) ex vivo when bioassayed on Nicotiana tabacum cv Samsun NN. CP-VIA-34 was thermostable and resistant to proteinase K. It did not exhibit protease (tested separately on BSA, and purified TMV), DNase and RNase activities. The paper discusses the characteristics of the CP-VIA-34, and its relevance in relation to some other known plant defence proteins such as the ribosome-inactivating proteins, inhibitor of virus replication, antiviral factor, and the PR-proteins.
- Subjects
GENETICS of disease resistance of plants; PAPAYA ringspot virus; RIBOSOME-inactivating proteins; ANTIVIRAL agents; VIRUS inhibitors; TOBACCO mosaic virus
- Publication
Journal of Plant Pathology, 2015, Vol 97, Issue 1, p45
- ISSN
1125-4653
- Publication type
Article